2009年

Kikuchi S, Saito Y, Ryto H, Fukunishi N, Abe T, Tanaka H, Tsujimoto H. Effect of heavy ion beams on chromosomes of common wheat, Triticum aestivum. Mutation Research - Functional and Molecular Mechanisms of Mutagenesis (in press).

Sato, H., D. Heang, H. Sassa and T. Koba: Identification and characterization of FT/TFL1 gene family in cucumber. Breed. Sci. 59:3-11(2009)

Manickavelu, A., T. Koba, K. Mishina and H. Sassa: Molecular characterization of crossabiltiy gene Kr1 for intergeneric hybridization in Trititcum aestivum (Poaceae: Triticeae). Plant Syst. Evol. 278: 125-131 (2009) (DOI 10.1007/s00606-008-0139-3, published on line: 14 Feb 2009)

Puerta, A.R., Ushijima, K., Koba, T. and Sassa, H. Identification and functional analysis of pistil-part self-incompatibility factor HT-B of Petunia. J Exp Bot 60: 1309-1318 (2009)

Puerta, A.R., Takagawa, M. and Sassa, H. Pistil non-S-specific self-incompatibility factor HT-B is tightly linked to the HT-like gene HTL in Petunia. Breed Sci 59: 99-101 (2009)

Kim, H., Kakui, H., Kotoda, N., Hirata, Y., Koba, T. and Sassa, H. Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.). Mol Breed 23: 463-472 (2009)

 

2008年

Kikuchi, S., Matsui, M., Tanaka, H., Ohnishi, O., Tsujimoto H.Chromosome evolution among seven Fagopyrum species revealed by
fluorescence in situ hybridization (FISH) probed with rDNAs. Chromosome Sci (in press)

Manickavelu, A., T. Koba, K. Mishina and H. Sassa: Identification of differential gene expression for Kr1 gene in bread wheat using annealing control primer system. Mol. Biol. Rep., DOI 10.1007/s11033-008-9423-0 (published on line:19 Dec. 2008)

Li, X. Z., X. J. Yuan, S. Jiang, J. S. Pan, S. L. Deng, G. Wang, H. L. He, A. Z. Wu, L. H. Zhu, T. Koba and R. Cai: Detecting QTLs for plant architecture traits in cucumber (Cucumis sativus L.). Breed. Sci. 58:453-460 (2008) (Accepted Oct. 28, 2008)

Sato, H., D. Heang, H. Sassa and T. Koba: The FT/TFL1 gene family in cucumber. Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, pp.603-607 (2008)

Heang, D., H. Sato, H. Sassa and T. Koba: Detection of two QTLs for fruit weight in cucumber (Cucumis sativus). Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, pp.511-514 (2008)

 

 

2007年

Kakui, H., Tsuzuki, T., Koba, T. and Sassa, H. (2007) Polymorphism of SFBB-g and its use for S genotyping in Japanese pear (Pyrus pyrifolia).  Plant Cell Rep  

Kim, H., Kakui, H., Koba, T., Hirata, Y. and Sassa, H. (2007) Cloning of a new S-RNase and development of a PCR-RFLP system for determination of the S-genotypes of Japanese pear.  Breed Sci 57: 159-164 

Sassa, H., Kakui, H., Miyamoto, M., Suzuki, Y., Hanada, T., Ushijima, K., Kusaba, M., Hirano, H. and Koba, T. (2007) S locus F-box brothers: Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear.  Genetics 175: 1869-1881  

Sassa, H. (2007) A technique to isolate DNA from woody and herbaceous plants by using a silica-based plasmid extraction column.  Anal Biochem 363: 166-167  

Ichida, H., Matsuyama, T., Abe, T. and Koba, T. (2007) DNA adenine methylation changes dramatically during establishment of symbiosis.  FEBS J 274: 951-962. 

Manickavelu, A., Kambara, K., Mishina, K. and Koba, T. (2007) An efficient method for purifying high quality RNA from wheat pistils. Colloids and Surfaces B. Biointerfaces 54: 254-258

Kikuchi S, Tanaka H, Wako T, Tsujimoto H. (2007) Centromere separation and association in the nuclei of an interspecific hybrid between Torenia fournieri and T. baillonii (Scrophulariaceae) during mitosis and meiosis. Genes Genet Syst. 82:369-375
[PubMed Link] [Article Link]

Kikuchi S, Kino H, Tanaka H, Tsujimoto H. (2007) Pollen Tube Growth in Cross Combinations between Torenia fournieri and Fourteen Related Species. Breed. Sci. 57:117-122
[PubMed Link] [Article Link]

 木庭卓人・狩野有香・石川正美　(2007) イチゴの減数分裂　−種子繁殖性イチゴ品種の育成に向けて−、　食と緑の科学 61:64-65.

 

 

2006年

 Azhaguvelu, p., D. Vidya-Saraswathi and T. Komatsuda (2006) High-resolution linkage mapping for the non-britle rachis locus btr1 in cultivated x wild type barley (Hordeum vulgare). Plant Sci. 170: 1087-1094

Ferdous, S. A., S. Watanabe, C. Suzuki-Orihara, Y. Tanaka, M. Kamiya, Z. Xia, N. Yamanaka, K. Harada (2006) Conversion of AFLP markers surrounding a QTL, rhg-t1,for soybean cyst nematode resistance into PCR-based markers. Breed. Sci. 56: 195-199.

Sandal, N., T. R. Peterson, K. Harada, J. Stougaard (2006) Genetics of symbiosis in Lotus japonicus:: Recombinant inbred lines, comparative genetic maps, and map position of 35 symbiotic loci. Mol. Plant-Microbe Interact. 19: 80-91

Sassa, H. and Hirano, H. (2006) Identification of a new class of pistil-specific proteins of Petunia inflata that is structurally similar to, but functionally distinct from, the self-incompatibility factor HT. Mol. Gen. Genom. 275: 97-104.

Tsubokura,Y., M. Hajika, K. Harada (2006) Molecular markers associated with β-conglycinin deficiency in soybean. Breed. Sci. 56: 155-163

Vidya-Saraswathi, D., P. Azhaguvelu, N. Senthil, T. Koba and T. Komatsuda (2006) Molecular mapping of non-brittle rachis gene btr1 and btr2 using STS markers in barley.Jpn. Agr. Res. Quar. 40: 239-242

 

 

2005年

 Kawaguchi, M., A. Pedrosa-Harand, K.Yano, M. Hay-ashi, Y. Murooka, K. saito, K. Namai, H. Nishida, D. Shi-bata, S. Sato, S. Tabata, M. Hayashi, K. Harada, N. Sandal, J. Stougaard, A. Bachmair and W. Grant(2005) Lotus burttii takes a position of the third corner in the lotus molecular genetics triangle.　DNA Res. 12: 69-77

Kubo, A., S. Rahman, Y. Utsumi, Z. LI,Y. Mukai, M. yamamoto. M, Ugai, H. satoh, C. Konik-Rose, M. Morell (2005) Complementation of sugary-1phenotype in rice endosperm with wheat isoamylase1gene supports a direct role for isomylase1 in amylopectin biosynthesis. Plant Physiol. 137: 43-56

Matumura, H., S. Watanabe, K. Harada, M. Senda, S. Akada, S. Kawasaki, E. G., Dubouzet, N. Minaka, R. Takahashi (2005) Molecular linkage mapping and phylogeny of the chalclone synthase multigene family in soybean. Theor. Apple. Genet. 110: 1203-1209

Oka-Kira, E., K. Tateno, K. Miura, T Haga, M. Hayashi, K. Harada, S. Sato, S. Tabata, N. shikazono, A. Tanaka, Y. Watanabe, I. Fukuhara, T. Nagata, M. Kawaguchi ( 2005) clavier (klv),a novel hypernodulation mutant of Lotus japonicus affected in vascular tissue organization and floral induction. Plant J. 44: 505-515

Arai M., M. Hayashi, M. Takahashi, S. Shimada and K. Harada (2005) Expression and sequence analysis of systematic regulation gene for symbiosis, NTS1/GmNARK in supernodulating soybean cultivar, Sakukei 4. Breed. Sci. 55: 147-152.

 Kawaguchi, M., Pedrosa-Harand, K. Yano, M. Hayashi, Y. Murooka, K. Saito, K. Namai, H. Nishida, D. Shibata, K. Saito, S. Tabata, M. Hayashi, K. Harada, N. Sandal, J. Stougaard, A. Bachmair and W. Grant (2005) Lotus burtii takes a position of sugary-1 phenotype in rice endosperm with wheat isoamylase1 gene supports a direct role for isoamylase1 in amyropectin biosynthesis. Plant Physiol. 137: 43-56.

 Kubo, A., S. Watanabe, K. Harada, M. Senda, S. Asada, S. Kawasaki, G., Dubouzet, N. Minaka and R. Takahashi (2005) Molecular linkage mapping and phylogeny of the chalcone synthetase multigene family in soybean. Theor. Appl. Genet. 110: 1203-1209.

 Nishioka, M., K. Tamura, M., Hayashi, Y. Fujimori, Y. Ohkawa, Y. Kuginuki and K. Harada (2005) Mapping of QTLs for bolting time in Brassica rapa (syn. campestris) under differnt enciromental conditions. Breed. Sci. 55: 127-133.

 Xia, Z., H. Sato, S. Watanabe, S. Kawasaki and K. Harada (2005) Construction and characterization of a BAC library of soybean. Euphytica 141: 129-137.

 Yamanaka, N., S. Watanabe, K. Toda, M. Hayashi, H. Fuchigami, R. Takahashi and K. Harada (2005) Fine mapping of FT1 locus for soybean flowering time, using a resiodual heterozygous line derived from a recombinant inbred line. Theor. Appl. Genet. 110: 634-639.

 

 

2004年

山本　亮・高橋良二・原田久也・高橋　幹・島田信二（2004）
根粒超着生変異品種作系4号の親子鑑定　育種学研究6：149-151

原田久也・穴井豊昭　（２００３）大豆種子で発現する遺伝子の同定と連鎖分析　大豆たん白質研究６：１８

Harada, K. and Xia, Z. (2004)
Soybean Genomics: Efforts to reveal the complex genome. Breed. Sci. 54: 215-224

Watanabe, S., Tajuddin T., Yamanaka N., Hayashi M and Harada K.(2004)
Analysis of QTLs for Reproductive Development and Seed Quality Traits in Soybean using Recombinant Inbred Lines. Breed. Sci. 54: 399-407

Yamanaka N., Watanabe S., Toda K., Hayashi M., Fuchigami H., Takahashi R., and Harada K.(2004)
Fine Mapping of FT1 Locus for Soybean Flowering Time, using a Residual Heterozygosity Line Derived from a Recombinant Inbred Line. TAG. 110: 634-639

Xia Z., Sato H., Watanabe S., Kawasaki S., and Harada K.(2004)
Construction and Characterization of a BAC Library of soybean. Euphytica 141: 129-137

 Tamura, K., M. Nishioka, M. Hayashi, Z. Zhand, C. Lian, T. Hougetsu and K. Harada (2004) Development of microsatellite markers by ISSR-suppression-PCR method in Brassica rapa. Breed. Sci. 55: 247-252.

 Watanabe, S., T. Tajuggin, N. Yamanaka, M. Hayashi and K. Harada (2004) Analysis of QTLs for reproductive development and seed quality traits in soybean using recombinant inbred lines. Breed. Sci. 54: 399-407.

 

 

2003年


Akasaka, M., Ueda, Y. and Koba, T.
Karyotype analyses of five wild rose species Belonging to septet B, C, and D by molecular cytogenetic method. breed. Sci. 53: 177-182

Hossin Md. S., Koba, T. and Harada, K.
Cloning and characterization of two full-length cDNAs TaGA1 and TaGA2, encoding G-protein α subunits expressed differentially in wheat genome. Genes and Genet. Syst. 78: 127-138

Hossin Md. S., Koba, T. and Harada, K.
Cloning and characterization of cDNA (TaGA1) encoding β subunit of heterotrimeric G-Protein from common wheat cv. S615. Plant Biotech. 20: 153-158

Tajuddin, T., Watanabe, S., Yamanaka, N. and Harada, K.
Analysis of quantative trait loci for protein and lipid contents in soybean seeds using recombinant inbred lines. Breed. Sci. 53: 133-140

Wong, K.-S., Kubo, A., Jane, J.-L., Harada, K., Satoh, H. and Nakamura Y.
Structures and properties of amylopectin and phytoglycogen in the endosperm of sugary-1 mutants of rice. J. Ceral Sci. 37: 139-149

原田久也・山中直樹
ダイズのDNAマーカー開発とその利用　わが国における食用マメ類の研究　66-73 中央農業総合センター

Harada, K., Hayashi, M. and Sato, S.
Genetic linkage map of the model legume Lotus japonicus. Biotechnology in Argriculture and Forestry Vol. 52 Brassicas and Legumes 167-182

川口正代司・原田久也・河内宏・中村保一・田畑哲之
モデル植物：ミヤコグサ.　植物の形づくり　1482-1487　共立出版

原田久也　農林水産生物研究に浸透するゲノム科学―第10回植物・動物・微生物・ゲノム国際会議報告―. 学術の動向　8:80-82

Hayashi, M., Aoki, T., Isobe, S., Harada, K., Kouchi, H., Minamisawa, K., Saeki, K., Sato, S., Tabata, S. and Kawaguchi, M.
A domestic weed goes worldwide. Recent progress on Lotus Research in Japan. Plant physiol. 131:840-842

 

2002年


Nishimura R., Hayashi M., Wu G.J., Kouchi H., Imaizumi-Anraku H., Murakami Y., Kawasaki S., Akao S., Ohmori M., Nagasawa M., Harada K., Kawaguchi M.
HAR1 mediates systemic regulation of symbiotic organ development.
Nature, 420(6914), 426-429.

Tajuddin, T., Watanabe, S., Masuda, R.., Harada, K., Kawano, S.
Application of near infrared transmittance spectroscopy to the estimation of protein and lipid contents in single seeds of soybean recombinant inbred lines for quantitative trait loci analysis. J. Near Infrared Spectrosc. 10: 315-325

Toda, K., D. Yang, N. Yamanaka, S. Watanabe, K. Harada and R. Takahashi
A single-base deletion in soybean flavonoid 3-hydroxylase gene is associated with gray pubescence color.
Plant Mol. Biol., 50, 187-196.

 

2001年


Hayashi, M., A. Miyahara, S. Sato, T. Kato, M. Yoshikawa, M. Taketa, M. Hayashi, A. Pedrosa, R. Onda, H. Imaizumi-Anraku, A. Bachmair, N. Sandal, J. Stougaard, Y. Murooka, S. Tabata, S. Kawasaki, M. Kawaguchi, K. Harada
Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F2 population.
DNA Research, 31, 301-310.


Onodera, Y., A. Suzuki, C.Y. Wu, H. Washida and F. Takaiwa
A rice functional transcriptional activator, RISBZ1, responsible for endosperm-specific expression of storage protein genes through GCN4 motif.
J Biol. Chem., 276, 14139-14152.


Yamanaka, N., S. Ninomiya, M. Hoshi, Y. Tsubokura, M. Yano, Y, Nagamura, T. Sasaki and K. Harada
An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion.
DNA Resarch, 8, 61-72.

 

2000年


Hayashi, M., M. Nishioka, K. Kitamura and K. Harada
Identification of AFLP markers tightly linked to the gene for deficiency of the 7S globulin in soybean seed and characterization of abnormal phenotypes involved in the mutation.
Breed. Sci., 50, 123-129.


Hossain, K.G., H. Kawai, M. Hayashi, M. Hoshi, N. Yamanaka and K. Harada
Characterization and identification of (CT)n microsatellites in soybean using sheared genomic libraries.
DNA Research, 7, 103-110.


Ito, M., N. Ohmido, Y. Akiyama, K. Fukui and T. Koba
Characterization of spinach chromosomes by condensation patterns and physical mapping of 5S and 45S rDNAs by FISH.
J. Amer. Soc. Hort. Sci., 125 (1), 59-62.


Kubo, N., X. Jordana, K. Ozawa, S. Zanlungo, K. Harada, T. Sasaki and K. Kadowaki
Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5’ untranslated region followed by gene duplication.
Mol. Gen. Genet., 263, 733-739.


Nozaki, T., K. Mishiba, M. Mii and T. Koba
Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campesteis.
Theor. Appl. Genet., 101, 538-546.


Touno, K., K. Harada, K. Yoshimatsu, K. Yazaki and K. Shimomura
Shikonin derivative formation on the stem of cultured shoots in Lithospermum erythrorhizon.
Plant Cell Reports, 19, 1121-1126.


Touno K., K. Harada, K. Yoshimatsu, K. Yazaki and K. Shimomura
Histological observation of red pigment formed on shoot stem of Lithospermum erythrorhizon.
Plant Biotechnology, 17, 127-130.


Wu, C-Y., H. Washida, Y. Onodera, K. Harada, F. Takaiwa
Quantitative nature of the prolamine-box, ACGT and AACA motifs in a rice glutelin gene promoter: minimal cis-element requirements for endosperm-specific gene expression.
Plant J., 23, 415-421.


Yamanaka, N., Y. Nagamura, Y. Tsubokura, K. Yamamoto, R. Takahashi, H. Kouchi, M. Yano, T. Sasaki and K. Harada
Quantitative trait locus analysis of flowering time in soybean using a RFLP linkage map.
Breed. Sci., 50, 109-115.


矢野博,原田久也
イネ培養変異のAFLP解析
DNA多型, 8, 79-82.

 

1999年


Aziz-purwantoro, K. Supaibulwatana, M. Mii and T. Koba
Cytological and RAPD (Random Amplified Polymorphic DNA) analyses of somaclonal variation in Easter Lily (Lilium longiflorum).
Plant Biotechnology, 16, 247-250.


Fujita, N., A. Kubo, P. B. Francisco, Jr., M. Nakakita, K. Harada, N. Minaka and Y. Nakamura
Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice.
Planta, 208, 283-293.


Kubo, A., N. Fujita, K. Harada, T. Matsuda, H. Satoh and Y. Nakamura
The starch-debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endsperm.
Plant Physiology, 121, 399-410.


Kubo, N., K. Harada, A. Hirai and K. Kadowaki
A single nuclear transcript encoding mitochondorial RPS14 and SDHB of rice is processed by altenative splicing:Commom use of the same mitochondrial targeting signal for different proteins.
Proc. Natl. Acad. Sci. USA, 96, 9207-9211.


Kubo, N., K. Harada and K. Kadowaki
Transfer of an rps11 gene from mitochondorion to the nucleus in pea.
Proceedings of International Congress on Plant Mitochondria, 25-27.


Washida, H., CY Wu, A. Suzuki, U. Yamanouchi, T. Akihama, K. Harada and F. Takaiwa
Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene GluB-1.
Plant Mol. Biol., 40, 1-12.


矢野博・原田久也
AFLP法による植物ゲノムDNAフィンガープリンティング
DNA多型, 7, 219-222.

 

1998年


Aziz-purwantoro and T. Koba
Localization of rRNA genes in Lilium by fluorescence in situ hybridization and its comparison with silver staining.
Chromosome Sci., 2, 77-82.


Hayashi, M., K. Harada, T. Fujiwara and K. Kitamura
Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merrill).
Mol. Gen. Genet., 258, 208-214.


Suzuki, A., CY. Wu, H. Washida and F. Takaiwa
Rice MYB protein OSMYB5 specifically binds to the AACA motif conserved among promoters of genes for storage protein glutelin.
Plant Cell Physiol., 39, 555-559.


Suzuki, T., M. Hayashi, Y. Iguchi and T. Koba
Isolation of cDNAs form pistils of common wheat, with special reference to crossability with alien species, Proc. 9th Intnatl. Wheat Genet. Symp., Saskatoon, Canada, 3, 66-68.


矢野博・原田久也
放射性PCR産物を用いた植物の放射線損傷DNAの解析
DNA多型, 6,136-141.


Wu, CY., A. Suzuki, H. Washida and F. Takaiwa
The GCN4 motif in a rice glutelin gene in essential for endosperm-specific gene expression and is activated by Opaque-2 in transgenic rice plants.
Plant J, 14, 673-683.