研究業績(2009年〜)

 

Kong K., Makabe S., Ntui V. O., Khan R. S., Nakamura I.

Synthetic chitinase gene driven by root-specific LjNRT2 and AtNRT2.1 promoters confers resistance to Fusarium oxysporum in transgenic tobacco and tomato.

Plant Biotecnol. Rep. in press


Kong K., Ntui V. O., Makabe S., Khan R. S. Mii M., Nakamura I.

Transgenic tobacco and tomato plants expressing Wasabi defensin genes driven by root-specific LjNRT2 and AtNRT2.1 promoters confer resistance against Fusarium oxysporum

Plant Biotech. 31, 89-96 (2014)


Yamaguchi T., Johzuka-Hisatomi Y., Terada R., Nakamura I., Iida S.

The MET1b gene encoding a maintenance DNA methyltransferase is indispensable for normal development in rice.

Plant Mol. Biol. 85, 319-232. (2014)


Ntui V., O., Kong K., Azadi P., Khan, R., S., Chin D., P., Igawa T., Mii M., Nakamura I.

RNAi-mediated resistance to cucumber mosaic virus (CMV) in genetically engineered tomato.

Amer. J. Plant Sci. 5, 554-572. (2014)


Mori T., Igawa T., Tamiya G., Miyagishima S., Berger F.

Gamete attachment requires GEX2 for successful fertilization in Arabidopsis 

Curr. Biol. 24, 170-175. (2014)


Ntui, V.O., K. Kynet, R.S. Khan, M. Ohara, Y. Goto, M. Watanabe, M. Fukami, I. Nakamura, M. Mii

Transgenic tobacco lines expressing defective CMV replicase-derived dsRNA are resistant to CMV-O and CMV-Y.

Mol. Biotechnol. 10.1007/s12033-013-9681-5. (2013)


Ntui, V.O., K. Kynet, P. Azadi, R.S. Khan, D.P. Chin, I. Nakamura, M. Mii

Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded

RNA modulates plant defense against CMV strains O and Y in potato.

Transgenic Res. 10.1007/s11248-013-9721-8. (2013)


Igawa T., Mori T.

Gamete membrane dynamics during double fertilization in Arabidopsis

Plant Signal Behav. 19:8(6). (2013)


Igawa T., Yanagawa Y., Miyagishima SY., Mori T.

Analysis of gamete membrane dynamics during double fertilization of Arabidopsis

Journal of Plant Research, 126, 387-394. (2013)


Wang, Y-P., C. Bounphanousay, K. Kanyavong, I. Nakamura, Y-I. Sato, T. Sato, H-S. Zhang, L-H. Tang, R. Ishikawa

Population structural analysis of in-situ conservation site for wild rice in Laos.

Genes Genet. Syst. 87: 311-322. (2012)


Khan, R. S., N. Kameya, M. Mii, I. Nakamura

Transgenic Petunia hybrida expressing a synthetic fungal chitinase gene confers disease tolerance to     Botrytis cinerea.

Plant Biotechnol. 29: 285-291. (2012)


Hoshikawa, K., G. lshihara, H. Takahashi, I. Nakamura

Enhanced resistance to gray mold (Botrytis cinerea) in transgenic potato plants expressing thionin genes isolated from Brassicaceae species.

Plant Biotech. 27: 87-93. (2012)


Hoshikawa, K, S. Endo, S. Mizuniwa, S. Makabe, H. Takahashi, I. Nakamura

Transgenic tobacco plants expressing endo-b-mannanase gene from deep-sea Bacillus sp. JAMB-602 strain confer enhanced resistance against fungal pathogen (Fusarium oxysporum)

Plant Biotech. Rep. 6: 243-250. (2012)


Rai, B., H. Takahashi, K. Kato, Y-I. Sato, I. Nakamura

Single-copy nuclear PolA1 gene sheds light on the origin of S genome with relationships to B and G genomes of polyploid wheat species.

Genet. Resour. Crop Evol. 59: 1713-1726. (2012)


Khan RS, Nakamura I, Mii M

Development of disease-resistant marker-free tomato by R/RS site-specific recombination.

Plant Cell Rep. 30: 1041-53. (2011)


Khan, R.S., S.S. Alam, I. Munir, P. Azadi, I. Nakamura, M. Mii

Botrytis cinerea-resistant marker-free Petunia hybrida produced using the MAT vector system.

Plant Cell Tiss. Organ Cult. 106: 11-20. (2011)


Azadi, P., V.O. Valentaine, S. Hasthanasombut, R.S. Khan, D.P. Chin, I. Nakamura, M. Mii

Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase

gene.

Biotech. Lett. 33: 1249-1255. (2011)


Khan, R.S., V.O. Ntui, D.P. Chin, I. Nakamura, M. Mii

Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker

Plant Cell Rep. 30: 587-597. (2010)


Zhan, L., D.P. Chin, M. Fukami, H. Ichikawa, I. Nakamura, M. Mii

Agrobacterium-mediated genetic transformation of Cattleya with an Odontoglossum ringspot virus replicase gene sequence.

Plant Biotech. 27: 421-426. (2010)


Khan, R.S., I. Nakamura and M. Mii

Production and selection of marker-free transgenic plants of Petunia hybrida using site-specific recombination.

Biol. Plantarum 54: 265-271. (2010)


Ntui, V.O., P. Azadi, G. Thirukkumaran, R.S. Khan, D.P. Chin, I. Nakamura, M. Mii

Increased resistance to fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes.

Plant Pathol. 60: 221-231. (2010)


Azadi, P., V. Ntui, D.P. Chin, I. Nakamura, M. Fujisawa, H. Harada, N. Misawa, M. Mii

Metabolic engineering of Lilium x formolongi using multiple genes of the carotenoid biosynthesis pathway.

Plant Biotech. Rep. 4: 269-280. (2010)


Hasthanasombut, S., K. Supaibulwatana, M. Mii and I. Nakamura

Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance.

Plant Cell Tiss. Organ Cult. 104: 79-89. (2010)


Ntui, V.O., G. Thirukkumaran, P. Azadi, R. S. Khan, I. Nakamura, M. Mii

Stable integration and expression of wasabi defensin gene in "Egusi" melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot.

Plant Cell Rep. 29: 943-954. (2010)


Ntui, V.O., R.S. Khan, D.P. Chin, I. Nakamura and M. Mii

An efficient Agrobacterium tumefaciens-mediated genetic transformation of Egusi melon (Colocynthis citrullus L.)

Plant Cell Tiss. Organ Cult. 103: 15-22. (2010)


Thirukumaran, G., V. O. Ntui, R. S. Khan, I. Nakamura, M. Mii

Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction.

Plant Biotech. 27: 147-153. (2010)


Khan, R.S., G. Thirukkumaran, I. Nakamura and M. Mii

Rol (root loci) gene as a positive selection marker to produce marker-free Petunia hybrida.

Plant Cell Tiss. Organ Cult. 101:279-285. (2010)


Hasthanasombut, S., V. Ntui, K. Supaibulwatana, M. Mii and I. Nakamura

Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco.

Plant Biotech. Rep. 4: 75-83. (2010)


Takahashi, H., B. Rai, K. Kato and I. Nakamura

Divergent evolution of wild and cultivated subspecies of Triticum timopheevii as revealed by the study of PolA1 gene.

Genet. Res. Crop Evol. 57:101-109. (2010)


Igawa T., Fujiwara M., Tanaka I., Fukao Y., Yanagawa Y.

Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants.
BMC Plant Biol. 10: 200 (2010)


Nakamura, I., B. Rai, H. Takahashi, K. Kato, Y-I. Sato

Aegilops section Sitopsis species contains the introgressive PolA1 gene with a closer relationship to that of Hordeum than Triticum-Aegilops species.

Breed. Sci. 59: 602-610. (2009)


Yamauchi, T., Y. Jozuka-Hisamori, S. Fukuda-Tanaka, R. Terada, I. Nakamura  and S. Iida.

Homologous recombination-mediated knock-in targeting of the MET1a gene for a maintenance DNA methyltransferase reproducibly reveals dosage-dependent spatiotemporal gene expression in rice.

Plant J. 60: 386-396. (2009)


Takahashi, H., T. Sato, Y.-I. Sato, I. Nakamura.

Genome-type-specific variation of the 19th intron sequence within the RNA polymerase I largest subunit gene in the genus Oryza.

Plant Syst. Evol. 282: 21-29. (2009)


Thirukkumaran, G., R.S. Khan, D.P. Chin, I. Nakamura, M. Mii.

Isopentenyl transferase gene expression offers the positive selection of marker-free transgenic plant of Kalanchoe blossfeldiana

Plant Cell. Tiss. Organ Cult. 97: 237-242. (2009)


Tsuchiya, T., N. Kameya, I. Nakamura

Straight Walk: A modified method of ligation-mediated genome walking for plant species with large genomes. Anal.

Biochem. 388: 158-160. (2009)


#Igawa T., Fujiwara M., Takahashi H., Sawasaki T., Endo Y., Seki M., Shinozaki K., Fukao Y., #Yanagawa Y.
Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings. 
J. Exp. Bot. 11, 3067-3073. (2009)

#These authors contributed equally to this work