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園芸学研究科　生物資源科学コース　応用生命化学領域

華岡　光正　准教授　博士（農学）
微生物工学特論、微生物資源化学

兵庫県西宮市生まれ。京都大学総合人間学部自然環境学科を卒業後、京都大学大学院修士課程を経て、2003年東京大学大学院農学生命科学研究科博士課程修了。「シロイヌナズナの葉緑体分化における転写システムの変換と転写制御の研究」により博士（農学）の学位を取得。日本学術振興会特別研究員DC1、科学技術振興機構CREST研究員、東京大学分子細胞生物学研究所博士研究員を経て、2008年千葉大学大学院園芸学研究科にテニュアトラックシステムの准教授として採用。千葉大学生命系科学推進室員を兼務。専門は植物生理学および分子生物学。植物に特有のオルガネラ（細胞小器官）である色素体に着目し、色素体遺伝子の転写制御、色素体分化の分子機構の解明を目指している。また、光合成微生物や高等植物における環境適応機構、特に光に応答した遺伝子発現調節、細胞内シグナル伝達のメカニズムについても研究を進めている。

主な業績

<原著論文>

• Yuki Kobayashi, Sousuke Imamura, Mitsumasa Hanaoka and Kan Tanaka (2011) A tetrapyrrole-regulated ubiquitin ligase controls algal nuclear DNA replication. Nature Cell Biol. 13, 483-487.
• Mitsumasa Hanaoka, Takayuki Kawakami and Kan Tanaka (2010) Chloroplast isolation and run-on transcription assay in Cyanidioschyzon merolae. Endocytobiosis Cell Res. 20, 45-52.
• Yuki Kobayashi, Mio Ohnuma, Tsuneyoshi Kuroiwa, Kan Tanaka and Mitsumasa Hanaoka (2010) The basics of cultivation and molecular genetic analysis of the unicellular red alga Cyanidioschyzon merolae. Endocytobiosis Cell Res. 20, 53-61.
• Yu Kanesaki, Yuki Kobayashi, Mitsumasa Hanaoka and Kan Tanaka (2009) Mg-protoporphyrin IX Signaling in Cyanidioschyzon merolae: Multiple pathways may involve the retrograde signaling in plant cells. Plant Signal. Behav. 4, 1190-1192.
• Mitsumasa Hanaoka and Kan Tanaka (2008) Dynamics of RpaB-promoter interaction during high light stress, revealed by chromatin immunoprecipitation (ChIP) analysis in Synechococcus elongatus PCC 7942. Plant J. 56, 327-335.
• Sousuke Imamura, Mitsumasa Hanaoka and Kan Tanaka (2008) The plant-specific TFIIB-related protein, pBrp, is a general transcription factor for RNA polymerase I. EMBO J. 27, 2317-2327.
• Asako Seki, Mitsumasa Hanaoka, Yuki Akimoto, Susumu Masuda, Hideo Iwasaki and Kan Tanaka (2007) Induction of a group 2 sigma factor, RPOD3, by high light and the underlying mechanism in Synechococcus elongatus PCC7942. J. Biol. Chem. 282, 36887-36894.
• Masahiko Imashimizu, Mitsumasa Hanaoka, Asako Seki, Katsuhiko Murakami and Kan Tanaka (2006) The DNA-binding role of the principal s factor region 1.1 in a cyanobacterium Thermosynechococcus elongatus BP-1. FEBS Lett. 580, 3439-3444.
• Mitsumasa Hanaoka, Kengo Kanamaru, Makoto Fujiwara, Hideo Takahashi and Kan Tanaka (2005) Glutamyl-tRNA mediates a switch in RNA polymerase usage during chloroplast biogenesis. EMBO Rep. 6, 545-550.
• Ayumi Minoda, Kirina Nagasawa, Mitsumasa Hanaoka, Makoto Horiuchi, Hideo Takahashi and Kan Tanaka (2005) Microarray Profiling of Plastid Gene Expression in a Unicellular Red Alga, Cyanidioschyzon merolae. Plant Mol. Biol. 59, 375-385.
• Mitsumasa Hanaoka, Shinya Murakami, Kazuya Morikawa, Takashi Shiina and Yoshinori Toyoshima (2004) Transcriptional regulation of the plastid gene expression in higher plants- A direct evidence for Sig1 to recognize the psbA promoter in wheat. Endocytobiosis Cell Res. 15, 209-217.
• Akitomo Nagashima, Mitsumasa Hanaoka, Makoto Fujiwara, Reiko Motohashi, Motoaki Seki, Kazuo Shinozaki, Kengo Kanamaru, Hideo Takahashi and Kan Tanaka (2004) DNA microarray analysis of plastid gene expression in an Arabidopsis mutant deficient in a plastid transcription factor sigma, SIG2. Biosci. Biotechnol. Biochem. 68, 694-704.
• Akitomo Nagashima, Mitsumasa Hanaoka, Makoto Fujiwara, Toshiharu Shikanai, Kengo Kanamaru, Hideo Takahashi and Kan Tanaka (2004) The multi-stress responsive plastid sigma factor, SIG5, directs activation of the psbD light responsive promoter (LRP) In Arabidopsis thaliana. Plant Cell Physiol. 45, 357-368.
• Mitsumasa Hanaoka, Kengo Kanamaru, Hideo Takahashi and Kan Tanaka (2003) Molecular genetic analysis of chloroplast gene promoters dependent on SIG2, a nucleus-encoded sigma factor for the plastid-encoded RNA polymerase, in Arabidopsis thaliana. Nucleic Acids Res. 31, 7090-7098.
• Yasunari Ogihara, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri and Koichiro Tsunewaki (2002) Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA. Mol. Genet. Genomics. 266, 740-746.
• Yasunari Ogihara, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri and Koichiro Tsunewaki (2000) Chinese Spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones. Plant Mol. Biol. Rep. 18, 243-253.

<総説、著書>

• Mitsumasa Hanaoka and Kan Tanaka (2010) Coordination of nuclear and plastid gene expression in red algae and green plants. In Red Algae in the Genomic Age, Joseph Seckbach and David Chapman (eds.), Springer, Berlin, Germany, pp. 171-190.
• 今村壮輔、華岡光正、田中寛（2009）植物で明らかにされたリボソームRNA合成酵素の進化の道筋—植物特異的なTFIIB型基本転写因子pBrpの機能解析—化学と生物、Vol.47, No.11, pp.740-742.
• 華岡光正、田中寛（2004）光と葉緑体分化プログラム　岡穆宏、岡田清孝、篠崎一雄編「植物の環境応答と形態形成のクロストーク」シュプリンガー・フェアラーク東京、pp.35-44.